CDM PreDictor Benzamidine 4FF HS

• Cat#No#: CD-061P
• Product Overview: HiTrap Benzamidine FF (high sub) are prepacked, ready to use 1 ml and 5 ml columns containing Benzamidine Sepharose 4 Fast Flow (high sub). These products are excellent tools for removal of proteolytic activity from a protein or peptide preparation or purification of trypsin and trypsin-like serine proteases such as thrombin and enterokinase.
  HiTrap Benzamidine FF (high sub) is also convenient to use for removal of enzymes (e.g., thrombin) after cleavage of the tag from a recombinant fusion protein. Fast, simple and easy separations are provided by the combination of the prepacked column with a high binding capacity affinity medium that has good flow properties.
  HiTrap Benzamidine FF (high sub) columns can be operated with a syringe, pump or liquid chromatography system.
• Characteristic: Fast and convenient to use.
  Very high binding capacity.
  Simple operation with a syringe, a pump, an ÄKTA system, or other chromatography systems.
  Scaling-up is easily achieved by connecting several 1 ml or 5 ml columns in series or using lab packages.
• Matrix: highly cross-linked 4% spherical agarose
• Ligand: p-aminobenzamidine (pABA)
• Ligand density: ≥ 12 µmol p-aminobenzamidine/ml medium
• Dynamic binding capacity: ≥ 35 mg trypsin/ml medium
• Recommended flow rate: 1 ml/min and 5 ml/min for 1 and 5 ml columns respectively.
• Chemical stability: All commonly used aqueous buffers
• pH working range: 1 to 9
• CIP stability: 2 to 8
• Storage: 4 to 8°C, 20% Ethanol
• Cleaning-in-place: The cleaning protocol has to be designed for each protein. General recommendation is to use a solution of guanidine hydrochloride to remove precipitated or denatured substances. For hydrophobically bound substances a solution of nonionic detergent or ethanol is recommended. Short term use of pH 1 to 9 is possible. For example, more than half of the trypsin capacity is available after 1 week (168 h) contact time at pH 9 (Fig 3). However, prolonged exposure to pH greater than 8 and lower than 2 should be avoided due to hydrolysis of the ligand at high pH and decomposition of the matrix at low pH.
• Pack size: 4 × 96-well filter plates
• Maximum flow velocity: 4 ml/min and 20 ml/min for 1 and 5 ml columns respectively.
• Dimensions: 0.7 × 2.5 cm (1 ml)
• Column volume: 1 ml and 5 ml
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)
• Centrifugation force recommended: 100 x g (Sample Dependent)-500 x g (Sample Dependent)
• Centrifugation force max.: 700 x g
• Operating temp. max.: 30°C
• Operating temp. min.: 4°C