CHT Ceramic Hydroxyapatite XT Media

• Product Overview: CHT XT Media is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. It is fine-tuned for the removal of aggregates and process impurities.
• Optimum pH: 6.5–14
• Characteristic: Unique selectivity
  Efficient single-step clearance of aggregates and other impurities
  High physical and chemical stability
  Straightforward column packing
• Reconstitution: 0.4 to 0.5 M sodium phosphate, pH 7.0-7.5; 1 M trisodium phosphate, pH 11-12. If higher concentrations of sodium phosphate are needed, use 0.4-1.0 M potassium phosphate.
• Functional group: Ca2+, PO4, OH
• Average particle size: 40 ± 4 µm
• Dynamic binding capacity: 17–25 mg Lysozyme/g*
  >60 mg/ml mAb G** (pI 9.2)
  * At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
  ** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column
• Maximum backpressure: 10 bar (150 psi)
• Bulk density: 0.67 g/ml
• Recommended flow rate: 50–300 cm/hr
  Tested on a 0.4 x 10 cm column packed at 150 cm/hr
• Chemical stability: 1 N sodium hydroxide, 8 M Urea, 6 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile, 0.4–1.0 M phosphate buffer, 0.5 M sodium phosphate, pH 7, 1.0 M trisodium phosphate, pH 11–12
• Recommended column storage: 0.1 N sodium hydroxide
• Autoclavable: 121°C, 20 min in phosphate buffered saline, pH 7
• Storage: 0.1 N sodium hydroxide