Chelating Resin (Fast Flow)

• Product Overview: This immobilized metal affinity resin is prepared by covalently linking iminodiacetic acid (IDA) to highly cross-linked agarose. It provides three coordination sites and three ionic interaction sites, offering higher affinity for target proteins than IMAC agarose FF, which has four coordination but only two ionic sites. It is compatible with reducing agents such as DTT and β-mercaptoethanol. Selection depends on the protein characteristics.
• Matrix: 6% Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 45–165 μm
• Ligand: Iminodiacetic acid (IDA)
• Coupling chemistry: Metal chelation (iminodiacetic acid, IDA)
• pH working range: 3–13
• pH CIP range: 2–14
• Chemical stability: Stable in common aqueous solutions: 1 M NaOH, 8 M urea, 6 M guanidine HCl
• Maximum operating pressure: 0.3 MPa
• Maximum flow velocity: > 300 cm/h
• Recommended flow rate: 60–200 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Product features: Non-pre charged metal ion resin with iminodiacetic acid (IDA) ligand.
  Strong metal binding: Compatible with reducing agents (DTT, β-mercaptoethanol).
  High stability, specificity, and flow rate.