Chelating Sepharose Fast Flow immobilized metal affinity chromatography resin

• Cat#No#: Ch-359C
• Product Overview: Chelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity:
  Based on well-established Sepharose Fast Flow resins.
  High chemical stability enables proven CIP and sanitization protocols.
  Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Chelating Sepharose Fast Flow is a BioProcess resin for immobilized metal ion affinity chromatography (IMAC) of native and histidine-tagged fusion proteins. The resin is suitable for both laboratory-scale purification and large-scale use, and is well-established in several production processes.
• Characteristic: Wide range of purification applications.
  Choice of a variety of chelated metal ions.
  High salt concentrations can be used.
  BioProcess resin used in approved industrial processes.
  Straightforward scale-up to production columns.
  Withstands effective and rigorous cleaning-in-place (CIP) procedures.
• Maximum operating pressure: 0.1 MPa (1.0 bar, 14.5 psi)
• Metal ion capacity: 30-37 µmol Cu₂₊/ml
• Packing Column: Chelating Sepharose Fast Flow is supplied preswollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replacing it with ultra pure water in a ratio of 75% settled resin to 25% ultra pure water.
• Matrix: 6% cross-linked agarose
• Ion exchange capacity: 30–37 µmol Cu2+/mL resin
• Functional group: pH:4 to 8.5
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 µm
• Ligand: Iminodiacetic acid
• Recommended flow rate: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1 M NaOH, 20% ethanol, 8 M guanidine hydrochloride, 8 M urea, 0.01 M HCl, non-ionic detergents, acetate buffer pH 4.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 40°C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Removing ionically bound proteins by washing the column with 0.5 column volumes of a 2 M NaCl solution.
  Removing precipitated proteins, hydrophobically bound proteins, and lipoproteins by washing the column with 1.0 M NaOH solution.
  Removing strongly hydrophobically bound proteins, lipoproteins, and lipids by washing the column with 70% ethanol or detergents in a basic or acidic solution.
• Sanitization: Sanitization reduces microbial contamination of the resin. A recommended sanitization procedure is treatment with 0.5 to 1.0 M NaOH.
• Pack size: 50 mL
• BioProcess resin: Yes