Dextrin Agarose 6 Fast Flow

• Cat#No#: AC-035M
• Product Overview: Dextrin Agarose 6FF is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).
  Dextrin is the polymeric product resulted by enzymatic hydrolysis of amylose with beta amylase. Compared to Amylose that is susceptible to amylase degradation, Dextrin is very stable. When used as the ligand substrate immobilized on the resin for purification of the MBP tag, Dextrin offers consistently strong affinity absorption even after repeated uses.
  The base matrix of Dextrin Agarose 6 Fast Flow is a robust, highly cross- linked 6% agarose. The crosslinking of the base matrix has been optimized to give high physical and chemical stability, high specificity of the binding.
  Affinity purification using Dextrin Agarose 6 Fast Flow take place under physiological conditions and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.
  Our proprietary coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.
• Characteristic: High selectivity for high purity
  > 10 mg MBP tagged protein (80 kDa)/mL medium
  Stable coupling of ligand, low leakage; can be re-used to purify the same protein
• Matrix: 6% highly cross-linked agarose supplied as 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Dextrin
• Dynamic binding capacity: >10 mg MBP tagged protein (80 kDa)/mL medium
• Recommended flow rate: 300 - 500 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar, 43 psi)
• pH working range: 3-12
• Storage: 2 - 8°C, storage buffer: 20% ethanol as preservative