CM Sephadex C-25

• Cat#No#: CM-438C
• Product Overview: CM Sephadex C-25 is a weak cation exchanger based on the well documented and well proven Sephadex base matrix.
  Weak cation exchanger suitable batch techniques.
  High binding capacities.
• Description: Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
• Metal ion capacity: 4-5 mmol/g
• Matrix: Cross-linked dextran
• Ionic Exchanger Type: Weak cation
• Particle Size: 40 µm-120 µm
• Ligand: Carboxymehyl
• Dynamic binding capacity: lgG (Mr 160 000)- 1.6 mg/mL drained resin), Bovine COHb (Mr 69 000)- 70 mg/mL drained resin), Ribonuclease (Mr 13 700)- 190 mg/mL drained resin)
• Recommended flow rate: ≥ 120 cm/h
• Chemical stability: Stable to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride.
• Physical stability: Negligible volume variation due to changed in pH or ionic strength.
• pH working range: 6–10
• CIP stability: 2–13
• Autoclavable: 30 min at 121°C in 0.1 M sodium chloride.
• Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
• Cleaning-in-place: Remove ionically bound proteins by washing the column with 0.5 to1 bed volume of a 2 M NaCl solution.
  Remove precipitated proteins, hydrophobically bound proteins and lipoproteins by washing the resin with 1 bed volume of a 0.1 M or 0.001 M NaOH.
  Strongly hydrophobically bound proteins, lipoproteins and lipids can be removed by washing the resin with up to 70% ethanol or 30% isopropanol.
  Alternatively, wash the resin with 2 bed volumes of detergent in a basic or acidic solution. After treatment with detergent always remove residual detergents by washing with 5 bed volumes of 70% ethanol.
• Pack size: 100 g
• BioProcess resin: No