CM Sephadex C-50

• Cat#No#: CM-439C
• Product Overview: CM Sephadex C-50 is a weak cation exchanger based on the well documented and well proven Sephadex base matrix.
  Weak cation exchanger suitable batch techniques.
  High binding capacities.
• Description: Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
• Metal ion capacity: 4-5 mmol/g
• Matrix: Cross-linked dextran
• Ionic Exchanger Type: Weak cation
• Ligand: Carboxymethyl
• Dynamic binding capacity: lgG (Mr 160 000)- 7 mg/mL drained resin), Bovine COHb (Mr 69 000)- 140 mg/mL drained resin), Ribonuclease (Mr 13 700)- 120 mg/mL drained resin).
• Recommended flow rate: ≥ 100 cm/h
• Chemical stability: Stable in water, salt solutions, organic solvents, alkaline and weakly acidic solutions and denaturing solvents.
• Physical stability: Volume changes due to changes in pH or ionic strength.
• pH working range: 6–10
• CIP stability: 2–12
• Autoclavable: 30 min at 121°C in 0.1 M sodium chloride.
• Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH.
• Cleaning-in-place: Depending of the nature of the sample, regeneration is normally performed by washing with a high ionic strength buffer (e.g., 1 to 2 M NaCl) and/or decreasing/increasing pH, followed by re-equilibration in binding buffer.
  In some applications, substances such as denaturated proteins or lipids do not elute in the regeneration procedure. These can be removed by cleaning procedures, CIP (Cleaningin-place).
  Strongly hydrophobically bound proteins, lipoproteins and lipids can be removed by washing the resin with up to 70% ethanol or 30% isopropanol.
  Alternatively, wash the resin with 2 bed volumes of detergent in a basic or acidic solution. Use for example, 0.1% to 0.5% nonionic detergent in 0.1 M acetic acid. After treatment with detergent always remove residual detergents by washing with 5 bed volumes of 70% ethanol.
• Pack size: 100 g
• BioProcess resin: No