CNBr-activated 4% agarose is cyanogen bromide pre-activated agarose gel, covalently couples biomolecules with primary amines (-NH2), suitable for affinity media and enzyme immobilization. Features: mild reaction conditions, direct coupling without spacer, multi-point coupling, stable ligands, applicable to proteins, peptides, nucleic acids.
Matrix
4% Agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
45–165 μm
Ligand
Primary amino group
Dynamic binding capacity
25–60 mg α-Chymotrypsinogen / mL resin
Coupling chemistry
CNBr activation
pH working range
3–11
pH CIP range
2–11
Chemical stability
Stable in all common aqueous solutions: 8 M urea, 6 M guanidine HCl, 70% ethanol
Maximum operating pressure
0.16 bar
Maximum flow velocity
≥ 15 cm/h
Pack size
25 mL; 100 mL; 500 mL
Storage
2–8 °C, 100% Acetone
Product features
Mild reaction conditions with proteins and biomolecules; allows direct coupling without spacer arms; ligand can form multi-site coupling with matrix/base, minimizing detachment; applied for coupling of proteins, peptides, and nucleic acids.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!