CNBr-Activated Sepharose 4 Fast Flow

• Cat#No#: CN-348C
• Product Overview: CNBr-activated Sepharose 4 Fast Flow is a well established, pre-activated chromatography resin for coupling of large amino-containing ligands:
  CNBr-activated BioProcess resin designed for coupling of large amino-containing ligands.
  Easy, rapid and efficient coupling.
  This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage.
  BioProcess resin supported for industrial applications and well-established in approved processes.
• Description: CNBr-activated Sepharose 4 Fast Flow is based on the well established Sepharose Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.
  The preparation and use of affinity chromatography resins by coupling biospecific ligands to CNBr-activated Sepharose 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.
  CNBr-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, CNBr-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
• Characteristic: Rapid, efficient coupling and maintained biological activity of the ligand.
  Multipoint attachment of many protein ligands, resulting in a chemically stable product.
  Fast Flow matrix gives high productivity and is easy to scale up.
  Comprehensive technical and regulatory supportfor pharmaceutical production simplifies validation.
  Overtwenty successful years of using CNBr-activated Sepharose media documented with many references.
• Maximum operating pressure: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Matrix: 4% cross-linked agarose
• Swelling: 4–5 ml drained gel/g
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Cyanogen bromide activated
• Recommended flow rate: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment.
• pH working range: 3–11
• CIP stability: 2–11 (ligand dependent)
• Storage: Unsused: 2 to 8°C - Used: 2 to 8°C in 20% Ethanol
• Cleaning-in-place: Wash with 2 column volumes of 6 M guanidine hydrochloride. Wash substances immediately with at least 5 column volumes of sterile filtered binding buffer.Wash the column with 2 column volumes of a nonionic detergent (conc. 0.1% to 0.5%). Wash immediately with at least 5 column volumes of sterile filtered binding buffer. Wash the column with 3 to 4 column volumes of 70% ethanol. Wash immediately with at least 5 column volumes of sterile filtered binding buffer.
• Sanitization: Sanitization inactivates microbial contaminants in the packed column and related equipment. A specific sanitization protocol should be designed for each process according to the type of contaminants present and stability of coupled ligand. Following are generally recommended procedures. Equilibrate with a buffer consisting of 2% hibitane digluconate and 20% ethanol. Allow to stand for 6 hours, then wash with at least 5 column volumes of sterile binding buffer. or Equilibrate with 70% ethanol. Allow to stand for 12 hours, then wash with at least 5 column volumes of sterile binding buffer.
• Pack size: 10 g
• BioProcess resin: Yes
• Maximum flow velocity: 250 cm/h