Con A Sepharose 4B

• Cat#No#: Co-360C
• Product Overview: Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography resin for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose:
  Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups.
  Group-specific adsorbent for molecules containing sugars.
  Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups.
  Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes Con A coupled to Sepharose 4B via cyanogen bromide activation.
• Description: Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
• Available capacity: 20 to 45 mg porcine thyroglobulin/ml medium
• Maximum operating pressure: Base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
• Medium Preparation: Con A Sepharose 4B is supplied pre-swollen in 0.1 M acetate buffer pH 6 containing 1 M NaCl, 1 mM CaCl2 , 1 mM MnCl2 and 1 mM MgCl2 . 20% ethanol is added as a preservative. Wash the required amount of medium with 10 bed volumes of binding buffer to remove the preservative. Prepare a slurry with binding buffer in a ratio of 75% settled medium to 25% buffer. The binding buffer should not contain agents which significantly increase the viscosity. The column may be equilibrated with viscous buffers at reduced flow rates after packing is completed.
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: 4% Agarose
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 μm
• Ligand: Concanavalin A (Con A)
• Ligand density: 13 mg Con A/ml drained medium
• Recommended flow rate: < 75 cm/h at 25 ˚C, HR 16/10 column, 5 cm bed height
• Recommended column height: 10 cm
• Chemical stability: Stable to commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 4–9
• CIP stability: 4–9
• Storage: 2 to 8°C, 0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl₂, 1 mM MnCl₂ and 1 mM MgCl, 20% Ethanol
• Pack size: 5 mL
• Column i.d.: 5 cm