COVID-19 Spike protein RBD-coupled magnetic beads

• Cat#No#: COV-002A
• Common Name: Spike
• Product Overview: The Antigen pre Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the COVID-19 Spike protein RBD-coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as Cell stimulating, biopanning or Flow cytometric analysis.
• Description: The pre-coupled magnetic beads coupled with biotinylated COVID-19 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- COVID-19 antibody or ACE2 protein from cell or serum sample.
  The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results.
  This very first COVID-19 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose
• Characteristic: Coupled amount of RBD protein: 80μg(2mg beads)/400μg(10mg beads)
• Applications: This product is intended for immunocapture, cell stimulating, biopanning and flow cytometry.
  a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
  b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
  Cell stimulating
  In order to study some downstream mechanisms, this antigen coupled beads can be used to stimulate your target cells. It can be easily removed using a magnet when its work is done.
  Biopanning
  Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.
  Flow cytometric analysis
  This antigen coupled beads can be used as feeder cells (antigen-presenting cells) to run your flow cytometric analysis, but without the hassle of cell culture.
• Reconstitution: 2 mL/10mL ultrapure water (1mg beads/mL)
• Average particle size: 2 μm
• Dynamic binding capacity: > 200 nmol / mg beads
  > 40 μg anti-COVID-19 S1 antibody / mg beads
  > 40 μg ACE2 protein / mg beads
• Storage: Upon receipt, please store the Beads at -20˚C for 1 year in lyophilized state.
  Once the Beads reconstitution, please use it immediately.