DEAE Sephadex A-25

• Cat#No#: DE-449C
• Product Overview: DEAE Sephadex A-25 is a weak anion exchanger based on the well documented and well proven Sephadex base matrix.
• Description: DEAE Sephadex A-25 is weak anion exchangers. The ion exchange group is diethylaminoethyl which remains charged and maintains consistently high capacity below pH 9.
• Characteristic: Weak anion exchanger suitable batch techniques.
  High binding capacities.
• Metal ion capacity: 3-4 mmol/g
• Matrix: Cross-linked dextran
• Ionic Exchanger Type: Weak and strong anion
• Particle Size: 40 µm-120 µm
• Ligand: Diethylaminoethyl
• Dynamic binding capacity: thyroglobulin (Mr 669 000)- 1 mg/ml drained resin), HSA (Mr 68 000)- 30 mg/ml drained resin), α-lactalbumin (Mr 14 300)- 140 mg/ml drained resin).
• Recommended flow rate: ≥ 120 cm/h
• Chemical stability: Stable to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride.
• Physical stability: Negligible volume variation due to changed in pH or ionic strength.
• pH working range: 2–9
• CIP stability: 2–13
• Autoclavable: In 0.1 M sodium chloride at 121°C for 30 min.
• Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH.
• Cleaning-in-place: Remove ionically bound proteins by washing the column with 0.5 to1 bed volume of a 2 M NaCl solution.
  Remove precipitated proteins, hydrophobically bound proteins and lipoproteins by washing the resin with 1 bed volume of a 0.1 M or 0.001 M NaOH solution followed by binding buffer until free from alkali.
  Strongly hydrophobically bound proteins, lipoproteins and lipids can be removed by washing the resin with up to 70% ethanol or 30% isopropanol.
  Alternatively, wash the resin with 2 bed volumes of detergent in a basic or acidic solution. After treatment with detergent always remove residual detergents by washing with 5 bed volumes of 70% ethanol.
• Pack size: 100 g
• BioProcess resin: No