Dextrin Sepharose High Performance MBP-tagged protein purification resin

• Cat#No#: De-389C
• Product Overview: Dextrin Sepharose High Performance is a robust and stable affinity resin for purification of proteins tagged with maltose binding protein (MBP).
  Fast, one-step purification of MBP-tagged proteins.
  High-resolution purification and elution in narrow peaks, minimizing the need for further concentration steps.
  Can be used for repeated purification: can be easily regenerated using 0.5 M NaOH.
  Physiological conditions and mild elution preserve target protein activity.
• Description: Dextrin Sepharose High Performance is a chromatography medium for purifying recombinant proteins tagged with maltose binding protein (MBP). The chromatography medium is available in 25 ml and 100 ml lab packs and prepacked in 1 ml and 5 ml MBPTrap HP columns.
  Tagging proteins with MBP often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increases solubility, the tag is particularly useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).
• Characteristic: Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  Physiological conditions and mild elution preserve target protein activity.
  Compatible with commonly used aqueous buffers and easily regenerated using 0.5 M NaOH.
  Easy scale-up.
  Prepacked MBPTrap HP 1 ml and 5 ml columns offer convenience, save time, and ensure reproducible results.
• Sample preparation: Adjust the sample to the composition of the binding buffer. For example, dilute the sample with binding buffer or buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or Desalting PD10 column.
  To avoid clogging the column when loading large sample volumes, filter the sample through a 0.45 μm filter or centrifuge it immediately before application.
• Packing Column: Dextrin High Performance is supplied preswollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replacing it with distilled water in a ratio of 75% settled medium to 25% distilled water. Water is used as packing solution.
• Matrix: Highly cross-linked agarose, 6%
• Average particle size: ~34 µm
• Ligand: Dextrin
• Dynamic binding capacity: Approx. 7 mg MBP- ∆Sal/ml resin Approx. 16 mg MBP- βGal/ml resin
• Recommended flow rate: ≤ 150 cm/h
• Chemical stability: Stable in commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning).
• pH working range: > 7
• CIP stability: 2–13
• Storage: 2 to 8°C, 20% Ethanol
• Pack size: 25 mL
• Maximum flow velocity: < 300 cm/h
• Maximum operating backpressure: 0.3 MPa, 3 bar, 43.5 psi
• Dimensions: 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml)
• Column volume: 1 ml or 5 ml
• Column hardware pressure limit: 0.5 MPa, 5 bar, 70 psi