EAH Sepharose 4B

• Cat#No#: EA-361C
• Product Overview: EAH Sepharose pre-activated resin is used for coupling compounds containing carboxyl groups to Sepharose 4B through carbodiimide- based coupling via an 11-atom spacer arm. Stable carbodiimide-based coupling of carboxyl groups to Sepharose 4B via an 11-atom hydrophilic spacer arm permits very stable coupling
• Matrix: Agarose, 4%
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand density: 7–12 μmoles amino groups/mL drained matrix
• Recommended flow rate: < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
• Chemical stability: Stable to commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–14
• CIP stability: 3–14
• Temperature stability: 4 °C – 25 °C
• Storage: 4 to 8°C, 20% Ethanol containing 0.9% NaCl
• Pack size: 50 mL