ECH-Lysine Sepharose 4 Fast Flow

• Cat#No#: EC-362C
• Product Overview: ECH-Lysine Sepharose 4 Fast Flow is a group specific resin for isolation of plasminogen and plasminogen activator. It is based on highly crosslinked 4% agarose thus enabling rapid processing of large sample volumes
• Description: ECH-Lysine Sepharose 4 Fast Flow is a group specific medium for isolation of plasminogen and plasminogen activator. It is based on highly crosslinked 4% agarose thus enabling rapid processing of large sample volumes. L-Lysine is covalently bound to a long hydrophilic spacer arm attached to Sepharose 4 Fast Flow via a stable ether linkage. ECH-Lysine Sepharose 4 Fast Flow was designed for industrial purification of plasminogen and plasminogen activator.
• Maximum operating pressure: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Ligand Coupling Method: Amide linkage
• Matrix: 4% cross-linked agarose
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: L-lysine
• Ligand density: 13–18 µmol Lysine/ml drained medium
• Coupling chemistry: NHS
• Dynamic binding capacity: > 1.5 mg Plasminogen/mL drained resin
• Recommended flow rate: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Recommended column height: 25 cm
• Chemical stability: Stable to commonly used aqueous buffers: 1 M NaOH, 6 M guanidine hydrochloride.
• pH working range: 3–12
• CIP stability: 2–13
• Storage: 4 to 30°C, in 20% Ethanol and 0.05 M Sodium Acetate
• Cleaning-in-place: Prolonged exposure, i.e., several days, to pH greater than 13 or lower than 2 should be avoided due to hydrolysis of the ligand at high pH and decomposition of the matrix at low pH. Strongly bound proteins can be removed with urea or guanidine hydrochloride.
• Sanitization: Generally sodium hydroxide (0.1–1 M) alone or in combination with sodium chloride (0.5–3 M) or ethanol (20–70%) is an effective sanitization agent.
• Pack size: 500 mL
• BioProcess resin: No