Epoxy-Activated Sepharose 6B

• Cat#No#: Ep-363C
• Product Overview: Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
• Description: Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands. Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Other ligands can be coupled through hydroxyl, amino or thiol groups. The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. Epoxy-activated Sepharose 6B is formed by reacting Sepharose 6B with 1,4-bis (2,3- epoxy-propoxy-) butane.
• Applications: The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules.
• Maximum operating pressure: base matrix 100-200 cm/h, pressure drop cm H₂O/bed height=15, bed height 10 cm, 5 cm i.d.
• Medium Preparation: Epoxy-activated Sepharose 6B is supplied freeze-dried in the presence of additives. These additives must be washed away at neutral pH before coupling the desired ligand.
  Weigh out the required amount of freeze dried powder (1 g freeze-dried powder gives about 3.5 mL final volume of medium) and suspend it in distilled water. The medium swells immediately and should be washed for 1 hour on a sintered glass filter. Use approximately 200 mL distilled water per gram freeze-dried powder, added in several aliquots.
• Matrix: Agarose, 6%
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand density: 19 to 40 μmole/mL drained medium
• Recommended flow rate: < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
• Recommended column height: 10 cm
• Chemical stability: Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• Temperature stability: 20°C to 40°C
• Storage: 2 to 8°C
• Pack size: 15 g
• Group To be Coupled: -NH₂, -OH, or -SH
• Column i.d.: 5 cm