Factor Xa

• Cat#No#: Fa-390C
• Product Overview: The Glutathione S-transferase (GST) Gene Fusion System is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli.
  Enables the site-specific cleavage of fusion proteins containing an accessible Factor Xa protease recognition sequence.
  Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow.
• Description: The Glutathione S-transferase (GST) Gene Fusion System is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. The system consists of three major components: pGEX plasmid expression vectors, products for GST purification, and a variety of GST detection products. A series of site-specific proteases for cleavage of the GST tag complements the system. The GST affinity tag permits a mild purification process that does not affect a protein's native structure and function.
• Form: Lyophilized powder
• Characteristic: All pGEX vectors offer a tac promoter for chemically inducible, high-level expression.
  Mild, nondenaturing buffer compositions for isolation of active proteins.
  Affinity chromatography products based on Glutathione Sepharose media for one-step purification of samples from low microgram to gram scale.
  Convenient prepacked formats suitable for single samples or parallel screening of multiple cloning constructs.
  PreScission Protease, Thrombin, or Factor Xa recognition sites on pGEX vectors for cleaving the target protein from the fusion product.
  Easy detection of fusion protein using Anti-GST Antibody.
• Applications: Used for site-specific separation of the GST tag from proteins expressed using pGEX-X vectors
• Pack size: 400 units