GammaBind Plus Sepharose antibody purification resin

• Cat#No#: Ga-326C
• Product Overview: GammaBind Plus Sepharose is a protein G affinity chromatography resin for purifying immunoglobulins (IgGs) from a variety of species: Enhanced binding to rat and mouse IgG subclasses compared with GammaBind G Sepharose.
  Binds to all mouse, rat, and human IgG subclasses; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Lab-scale IgG purification yields up to 35 mg human IgG/mL.
  Well suited for immunoprecipitation procedures.
• Description: GammaBind Plus Sepharose is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by malimide linkage. This rigid matrix results in easy handling and fast separations.
  GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen. Hence, GammaBind Plus Sepharose is very useful for isolation of immune complexes
• Applications: GammaBind Plus Sepharose is used to analyze and purify classes, subclasses, and fragments of immunoglobulins from any biological fluid or cell culture medium. This resin is also suitable for immunoprecipitation.
• Medium Preparation: GammaBind Plus Sepharose is supplied preswollen in phosphate buffered saline (PBS), pH 7.0 containing 20% ethanol as preservative. Prepare a slurry by decanting the phosphate buffered saline solution and replace it with binding buffer, in a ratio of 75% settled medium to 25% buffer. The binding buffer should not contain agents which significantly increase the viscosity. The column may be equilibrated with viscous buffers at reduced flow rate after packing is completed.
  For batch procedures remove the phosphate buffered saline solution by washing the medium on a medium porosity sintered glass funnel.
• Ligand Coupling Method: Maleimide activation
• Matrix: Cross-linked agarose, 6%
• Average particle size: ~90 µm
• Ligand: Recombinant GammaBind G type 3 lacking albumin-binding region
• Ligand density: 3 mg GammaBind G, type 3/ml medium
• Coupling chemistry: malimide linkage
• Dynamic binding capacity: 35 mg human IgG/ml drained medium
  7 mg mouse IgG/ml drained medium
• Recommended flow rate: < 130 cm/h
• Chemical stability: Stable to all commonly used aqueous buffers and additives such as 1 M acetic acid, 1% SDS and 6 M guanidine hydrochloride.
• Physical stability: Negligible volume variation due to changes in pH or ionic strengt.
• pH working range: 3–9
• pH CIP range: 2–9
• Storage: 2 to 8°C, 20% Ethanol
• Notes: If you have packed at the maximum flow velocity, do not exceed 75% of this in subsequent chromatographic procedures.
• Cleaning-in-place: pH stability where the medium can be subjected to cleaning- or sanitization-in-place without significant change in function.
• Pack size: 5 mL
• Maximum flow velocity: 130 cm/h
• Maximum operating backpressure: 0.015 MPa (0.15 bar, 2 psi)