GammaBind Plus Sepharose resin

• Cat#No#: Ga-432C
• Product Overview: GammaBind Plus Sepharose is a protein G affinity chromatography resin for purifying immunoglobulins (IgGs) from a variety of species.
• Characteristic: Enhanced binding to rat and mouse IgG subclasses compared with GammaBind G Sepharose.
  Binds to all mouse, rat, and human IgG subclasses; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Lab-scale IgG purification yields up to 35 mg human IgG/mL.
  Well suited for immunoprecipitation procedures.
• Maximum operating pressure: 130 cm/h
• Medium Preparation: GammaBind Plus Sepharose is supplied preswollen in phosphate buffered saline (PBS), pH 7.0 containing 20% ethanol as preservative. Prepare a slurry by decanting the phosphate buffered saline solution and replace it with binding buffer, in a ratio of 75% settled medium to 25% buffer. The binding buffer should not contain agents which significantly increase the viscosity. The column may be equilibrated with viscous buffers at reduced flow rate after packing is completed.
  For batch procedures remove the phosphate buffered saline solution by washing the medium on a medium porosity sintered glass funnel.
• Ligand Coupling Method: Maleimide activation
• Matrix: Cross-linked agarose, 6%
• Average particle size: ~90 µm
• Ligand: Recombinant GammaBind G type 3 lacking albumin-binding region.
• Ligand density: 3 mg GammaBind G, type 3/ml medium
• Coupling chemistry: malimide linkage
• Dynamic binding capacity: 35 mg human IgG/ml drained medium; 7 mg mouse IgG/ml drained medium.
• Recommended flow rate: < 130 cm/h
• Chemical stability: Commonly used aqueous buffers.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–9
• CIP stability: 2–9
• Storage: 2 to 8°C, 20% Ethanol
• Sanitization: Sanitize the column with 70% ethanol
• Pack size: 5 mL
• Maximum operating backpressure: 0.015 MPa (0.15 bar, 2 psi)