Gelatin Sepharose 4B affinity resin with immobilized gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose 4B is bovine gelatin coupled to Sepharose 4B by the cyanogen bromide method
Description
Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma. Gelatin Sepharose 4B has been designed for the purification or removal of fibronectin.
Maximum operating pressure
75 cm/h
Ligand Coupling Method
Cyanogen bromide activation
Matrix
agarose, 6%, spherical
Average particle size
~90 µm
Ligand
Bovine gelatin
Ligand density
4.5-8 mg gelatin/ml drained resin
Recommended flow rate
< 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
Chemical stability
Stable to commonly used aqueous buffers
Physical stability
Negligible volume variation after changes in pH or the ionic strength.
pH working range
3–10
CIP stability
3–10
Storage
2 to 8°C, 20% Ethanol
Cleaning-in-place
In some applications, substances like denaturated proteins or lipids do not elute by the regeneration. These substances can be removed by washing the resin using a detergent solution, at 37°C for one minute. Re-equilibrate the resin then immediately with at least 5 bed volumes using the binding buffer.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!