Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications. The cartridge prepacked with Glutathione Agarose 4FF is ready-to-use, and is compatible with syringe, AKTA, and other peristaltic pump systems.
Matrix
4% highly cross-linked agarose
Average particle size
45 μm - 165 μm
Ligand
Glutathione coupled with a 12-atom linker
Ligand concentration
120 - 320 μmol Glutathione/mL medium
Dynamic binding capacity
>10 mg GST (26 kDa)/mL medium
Recommended flow rate
300 - 500 cm/hour
Maximum Pressure Drop
0.3 MPa (3 bar, 43 psi)
pH working range
3-12
Storage
2 - 8°C, storage buffer: 20% ethanol as preservative
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For Research or Industrial Raw Materials, Not For Personal Medical Use!