Glutathione Sepharose 4 Fast Flow resin Resin

• Cat#No#: Glu-394C
• Product Overview: Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
  Fast, one-step GST-tagged protein purification.
  Mild elution conditions preserve protein antigenicity and function.
  Excellent scale-up due to high flow properties.
• Description: The glutathione ligand is coupled via a 10-carbon linker to highly crosslinked 4% agarose. The coupling is optimized to give high binding capacity for GST-tagged proteins and other glutathione binding proteins.
  The total binding capacity is approximately 10 mg recombinant GST/mL medium. The dynamic binding capacity will vary depending on several factors such as target protein, flow rate etc.
• Maximum operating pressure: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Sample preparation: The sample should be centrifuged and/or filtered through a filter before it is applied to the medium.
  If the sample is too viscous, dilute it with binding buffer to prevent clogging the column. It is not necessary to filter the sample before performing batch .purification.
• Ligand Coupling Method: Epoxy activation
• Matrix: Highly cross-linked 4% agarose
• Average particle size: ~90 µm
• Ligand: Glutathione and 10-carbon linker arm
• Ligand density: 120 to 320 μmol glutathione/mL medium
• Dynamic binding capacity: ≈11 mg GST-tagged protein/mL medium Mr43 000 (GSTrap FF 1 mL at 1 mL/min=156 cm/h).
• Recommended flow rate: < 450 cm/h
• Recommended column height: 25 cm
• Chemical stability: Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature.
• pH working range: 3–12
• CIP stability: 3–12
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Removal of precipitated or denatured substances: Wash with 2 column volumes of 6 M guanidine hydrochloride, immediately followed by 5 column volumes of PBS, pH 7.3.
  Removal of hydrophobically bound substances: Wash with 3 to 4 column volumns of 70% ethanol or 2 column volumes of 1% Triton X-100, immediately followed by 5 column volumes of PBS, pH 7.3.
• Pack size: 25 mL
• Maximum flow velocity: 450 cm/h (15 mL/min), using XK 16/20 column with 5 cm bed height, run at room temperature with aqueous buffer.