Glutathione Sepharose High Performance resin Resin

• Cat#No#: Glu-396C
• Product Overview: Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority.
• Description: Glutathione Sepharose High Performance is an affinity chromatography medium designed for easy, one-step purification of glutathione Stransferase (GST) tagged proteins produced using the pGEX series of expression vectors, other glutathione S-transferases and glutathione binding proteins.
  GST-tagged proteins can be purified directly from pre-treated bacterial lysates using Glutathione Sepharose High Performance. The tagged proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and function.
• Characteristic: Fast, one-step GST-tagged protein purification.
  The small bead size (34 µm) enables high-resolution purification of tagged proteins.
  Mild elution conditions preserve protein antigenicity and function.
• Sample preparation: The sample should be centrifuged and/or filtered through a 0.45 μm filter immediately before it is applied to the medium. If the sample is too viscous, dilute it with binding buffer to prevent clogging the column.
• Packing Column: Glutathione Sepharose High Perfomance is supplied preswollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replacing it with distilled water in a ratio of 75% settled medium to 25% distilled water.
• Matrix: Highly crossed-linked agarose, 6%
• Average particle size: ~34 μm
• Ligand: Glutathione
• Dynamic binding capacity: >Approx. 10 mg GST-tagged protein/mL resin.
• Recommended flow rate: < 600 cm/h
• Chemical stability: Commonly used aqueous buffers
• pH working range: 3–12
• CIP stability: 3–12
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Removal of precipitated or denatured substances: Wash with 2 column volumes of 6 M guanidine hydrochloride, immediately followed by 5 column volumes of PBS, pH 7.3.
  Removal of hydrophobically bound substances: Wash with 3 to 4 column volumns of 70% ethanol or 2 column volumes of 1% Triton X-100, immediately followed by 5 column volumes of PBS, pH 7.3.
• Pack size: 25 mL
• Maximum flow velocity: 600 cm/h
• Maximum operating backpressure: 0.3 MPa (3 bar, 43 psi)
• Column volume: 0.4 mL