GST GraviTrap

• Cat#No#: GST-093P
• Product Overview: GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein.
• Description: GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. GST-tagged proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins.
• Characteristic: Convenient, prepacked gravity-flow columns.
  Allows direct purification of both clarified and unclarified cell lysates.
  Yields up to 20 mg of pure proteins per column.
• Applications: For fast and simple manual purification of GST-tagged proteins by gravity flow.
• Particle Size: 45 to 165 μm
• Ligand: Glutathione and 10-carbon linker arm
• Ligand density: 7 to 15 μmol glutathione/ml medium
• Dynamic binding capacity: Approx. 10 mg recombinant glutathione Stransferase (Mr 26 000)/mL medium (protein dependent)
• Chemical stability: No significant loss of the capacity is detected when Glutathione Sepharose 4B is exposed to 0.1 M citrate (pH 4.0), 0.1 M NaOH, 70% ethanol or 6 M guanidine hydrochloride2 for 2 hours at room temperature. No significant loss of binding capacity is observed after exposure to 1% SDS for 14 days.
• pH working range: 4 to 13
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 10 × 2 mL
• Column volume: 13 mL
• Material: Polypropylene barrel, polyethylene frits