GST MultiTrap 4B

• Cat#No#: GST-094P
• Product Overview: GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.
• Description: GST MultiTrap 4B is prepacked disposable 96-well filter plates for reproducible high-throughput screening of glutathione S-transferase (GST) tagged proteins. The 96-well filter plates are prepacked with Glutathione Sepharose 4B, respectively. GST-fusion proteins can be produced using the pGEX series of expression vectors.
  These filter plates simplify the purification screening and small scale purification of up to 0.5 mg of GST-tagged proteins/well. After thorough cell disruption, it is possible to apply unclarified lysate directly to the wells in the 96-well filter plate without precentrifugation and/or filtration of the sample. It is recommended to extend the duration of mechanical/chemical lysis if the sample is too viscous after lysis. The tagged proteins are eluted under mild, nondenaturing conditions that preserve protein antigenicity and function.
• Characteristic: 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
  Highly reproducible well-to well and plate-to-plate results.
  High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  Wells are prepacked with Glutathione Sepharose resins for convenience.
  Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.
• Average particle size: 90 μm
• Dynamic binding capacity: Up to 0.5 mg GST-tagged protein/well.
• Chemical stability: All commonly used aqueous buffers, e.g., 1 M acetate, pH 4.0 and 6 M guanidine hydrochloride for 1 hour at room temperature.
• CIP stability: 4 to 13
• Storage: 4 - 30°C, 20% Ethanol
• Pack size: 4 × 96-well filter plates
• Number of wells: 96
• Centrifugation force recommended: 100 x g-500 x g
• Centrifugation force max.: 700 x g