GST SpinTrap

• Cat#No#: GST-095P
• Product Overview: GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates.
• Description: GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.
• Characteristic: Preserves protein antigenicity and function.
  Yields up to 500 μg of pure protein per column.
  Allows direct purification of both clarified and unclarified cell lysates.
• Applications: For simple, small-scale purification of GST-tagged proteins and rapid expression screening by centrifugation.
• Average particle size: 90 μm
• Ligand: Glutathione and 10-carbon linker arm
• Ligand density: 7–15 μmol glutathione/mL medium.
• Dynamic binding capacity: Up to 500 µg recombinant glutathione S-transferase (rGST)
• Chemical stability: No significant loss of the capacity is detected when Glutathione Sepharose 4B is exposed to 0.1 M citrate (pH 4.0), 0.1 M NaOH, 70% ethanol or 6 M guanidine hydrochloride2 for 2 hours at room temperature. No significant loss of binding capacity is observed after exposure to 1% SDS for 14 days.
• CIP stability: 4 to 13
• Storage: 4 to 30°C, PBS and 0,05% Kathon CG/ICP Biocide.
• Pack size: 50 × 50 µL
• Column volume: 900 μL
• Material: Polypropylene barrel, polyethylene frits