GST-Tag Affinity Resin (Fast Flow)

• Product Overview: This high-crosslinked agarose affinity resin is prepared by immobilizing glutathione and is designed for specific purification of GST and GST fusion proteins. The medium supports mild operating conditions, fast flow, high pressure tolerance, and preserves protein activity, suitable for proteins from various sources.
• Matrix: 4% Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 45–165 μm
• Ligand: Glutathione with 10-atom spacer arm, 120–230 μmol GST/mL resin
• Dynamic binding capacity: ~10 mg GST / mL packed resin
• Chemical stability: Stable in common aqueous solutions: 1 M acetic acid, 70% ethanol, 6 M guanidine HCl (room temperature 1 h)
• Maximum operating pressure: 0.3 MPa
• Maximum flow velocity: ~450 cm/h
• Recommended flow rate: Sample loading flow rate: < 100 cm/h
  Equilibration, wash, and elution flow rate: 100–300 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Suitable for purification of GST-tagged proteins.
  High purity and pressure resistance: Mild operation maintains protein activity.
  High flow rate and scalable performance.