GSTrap HP Columns
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GSTrap HP Columns

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GST-099P GSTrap HP Columns Inquiry
Product Information
Cat#No# GST-099P
Product Overview GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Description GSTrap HP 1-mL and 5-mL columns are prepacked with Glutathione Sepharose High Performance resin, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity resin, and impurities are removed by washing with binding buffer.
Characteristic High resolution purification of GST-tagged proteins.
Mild elution conditions preserving protein antigenicity and function.
Fast, one-step purification.
Easy to use with syringe, pump or a chromatography system.
Applications Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins.
Maximum operating pressure 5 bar [0.5 MPa] (70 psi)
Recommended flow rate < 4 ml/min
Recommended column height 25 mm
Storage 4 to 30°C, 20% Ethanol
Pack size 5 × 1 mL
Dimensions 7 × 25 mm
Column volume 1 ml
Column i.d. 7 mm
Column hardware pressure limit 5 bar (0.5 MPa)
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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