GSTrap HP Columns

• Cat#No#: GST-099P
• Product Overview: GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
• Description: GSTrap HP 1-mL and 5-mL columns are prepacked with Glutathione Sepharose High Performance resin, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity resin, and impurities are removed by washing with binding buffer.
• Characteristic: High resolution purification of GST-tagged proteins.
  Mild elution conditions preserving protein antigenicity and function.
  Fast, one-step purification.
  Easy to use with syringe, pump or a chromatography system.
• Applications: Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)