Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
GST-099P | GSTrap HP Columns | Inquiry |
Product Information | |
Cat#No# | GST-099P |
Product Overview | GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. |
Description | GSTrap HP 1-mL and 5-mL columns are prepacked with Glutathione Sepharose High Performance resin, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity resin, and impurities are removed by washing with binding buffer. |
Characteristic | High resolution purification of GST-tagged proteins. Mild elution conditions preserving protein antigenicity and function. Fast, one-step purification. Easy to use with syringe, pump or a chromatography system. |
Applications | Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins. |
Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
Recommended flow rate | < 4 ml/min |
Recommended column height | 25 mm |
Storage | 4 to 30°C, 20% Ethanol |
Pack size | 5 × 1 mL |
Dimensions | 7 × 25 mm |
Column volume | 1 ml |
Column i.d. | 7 mm |
Column hardware pressure limit | 5 bar (0.5 MPa) |
Download Datasheet |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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