Heparin Sepharose 6 Fast Flow affinity resin

• Cat#No#: He-367C
• Product Overview: Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.
  Proven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors. Based on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
  Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.
  Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Heparin Sepharose 6 Fast Flow is a BioProcess resin for affinity chromatography (Fig 1). The resin allows fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. The excellent flow characteristics and high chemical stability of Heparin Sepharose 6 Fast Flow make the resin highly suitable for process-scale purifications. As a member of the BioProcess family of resins. Heparin Sepharose 6 Fast Flow is well-supported with documentation that facilitates development, scale-up, and routine operation in production applications.
• Characteristic: Purification of heparin-binding biomolecules.
  High chemical stability based on stable coupling chemistry.
  Enhanced binding capacity for antithrombin III due to oriented coupling of the ligand.
  Widely used in industrial applications and well-established in approved processes.
• Applications: Heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.
• Maximum operating pressure: 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)
• Ligand Coupling Method: Reductive Amination
• Matrix: cross-linked agarose, 6%, spherical
• Average particle size: ~90 µm
• Ligand: Porcine heparin
• Ligand density: ~4mg heparin/mL resin
• Recommended flow rate: 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 0.1 M NaOH, 0.05 M sodium acetate, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergents, 10% glycerol.
• pH working range: 4–12
• CIP stability: 4–13
• Autoclavable: 30 min at 121°C in 0.02 M NaH₂PO₄, pH 7, 5 cycles
• Storage: 4 to 30°C, 20% Ethanol, 0.05 M Sodium Acetate
• Shipping: 20% ethanol containing 0.05 M sodium acetate
• Cleaning-in-place: Heparin Sepharose 6 Fast Flow withstands exposure to 0.1 M NaOH for long periods without significant loss of binding capacity for antithrombin III. When contamination is severe, 0.5 M NaOH can be used. However, a decrease in functionality can be seen over time. Other reagents in which the resin is stable include 8 M urea and 6 M guanidine hydrochloride.
• Sanitization: Sanitization reduces microbial contamination of the resin. A recommended sanitization procedure is to treat the packed column with 0.1 M NaOH and 20% ethanol for 1 h. Always wash the packed column with equilibration buffer after sanitization.
• Pack size: 50 mL
• BioProcess resin: Yes
• Dimensions: 5 cm