High-Resolution Mixed-Mode Cation Exchange Resin

• Matrix: Highly Rigid Agarose
• Average particle size: ~60 μm
• Ligand: N-Benzoyl-DL-homocysteine
• Capacity: 60–100 µmol H⁺/mL
• Chemical stability: Stable in common aqueous solutions: 1 M NaOH, 8 M urea, 6 M guanidine HCl, 70% ethanol, 30% isopropanol, etc.
• Maximum flow velocity: 1500 cm/h
• Recommended flow rate: 150–300 cm/h
• pH working range: 3–12
• Storage: 2–30 °C in 20% ethanol
• Shelf life: 5 years
• Product Overview: Mixed-Mode Cation Exchange Resin is weak cation-exchange multimodal resins, prepared by coupling the ligand to a highly rigid agarose matrix with N-benzoyl-DL-homocysteine. The ligand contains multiple functional groups: carboxyl groups function as conventional weak cation exchangers, the phenyl ring provides hydrophobic interaction, the amide bond enables hydrogen bonding, and the sulfur atom offers unique selectivity. These interactions can operate independently or cooperatively, making the resins well-established for applications with antibodies, recombinant proteins, plasma proteins, and viral particles. This product uses highly rigid agarose microspheres that feature high flow rates, low back pressure, and high pressure resistance. Mixed-Mode Cation Exchange Resin has an average particle size of ~60 μm for easy scale-up, while Mixed-Mode Cation Exchange Resin HR has an average particle size of ~35 μm for higher resolution. Compared with conventional cation-exchange resins, MMC weak cation-exchange multimodal resins offer the following advantages:
  Multiple interaction modes enable effective target-protein binding even under high-salt conditions. While ionic interactions diminish at high salt, hydrophobic interactions and hydrogen bonding persist, allowing Mixed-Mode Cation Exchange Resin to bind proteins effectively in buffers with conductivity up to 30 mS/cm and at higher pH, improving speed and throughput in capture and intermediate purification steps.
  The synergy between ionic and hydrophobic interactions gives multimodal resins a different selectivity from cation-exchange resins. Adding modifiers such as urea and lyotropic salts to the mobile phase can significantly alter purification behavior, offering high operational flexibility.