| Product Information | |
| Cat#No# | Hi-308P |
| Product Overview | Designed for high-resolution protein fractionation at preparative scales. Pre-packed with Superdex 200 pg resin, these size exclusion chromatography columns enable purification of mAbs, DNA fragments and other biomolecules with Mr~ 10 000 to ~ 600 000. |
| Description | HiLoad Superdex 200 preparation grade resin is well suited for rapid, preparative protein purification and scale-up. Its selectivity curve gives a broad fractionation range from Mr ~ 10 000 to 600 000, with excellent resolving power for antibody monomers, dimers and contaminants of lower molecular weight, such as albumin and transferrin. |
| Characteristic | Preparative-grade: high purity and resolution. Two column sizes available: for samples up to 5 mL and up to 13 mL. Prepacked columns: convenient and reproducible; can be used with standalone pump or chromatography system. Scalable SEC: resin properties and column dimensions optimized for preparative-scale protein purification; choose from two XK column sizes (16/600 or 26/600). Compatible with ÄKTA systems: fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems. |
| Maximum operating pressure | 0.3 MPa (3 bar, 42 psi) |
| Matrix | Cross-linked agarose, spherical |
| Average particle size | ~ 34 μm |
| Recommended flow rate | 10 cm/h - 50 cm/h |
| Recommended column height | 600 mm |
| pH working range | 3 to 12 |
| CIP stability | 1 to 14 |
| Storage | 4 to 30°C, 20% Ethanol |
| Shipping | 20% ethanol |
| Cleaning-in-place | Wash the column with one-half to one CV 0.5 M NaOH at a flow rate of 0.8 mL/min for 16/600 or 2.2 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin. After cleaning, immediately equilibrate the column with at least two CV buffer. Further equilibration is necessary if the buffer contains detergents. Wait until the UV baseline stabilizes before starting a new purification. |
| Pack size | 1 × 120 mL |
| Dimensions | 16 × 600 mm |
| Column i.d. | 16 mm |
| Column hardware pressure limit | 0.5 MPa (5 bar, 73 psi) |
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