HiLoad Superdex 75 pg columns

• Cat#No#: Hi-310P
• Product Overview: HiLoad Superdex 75 pg prepacked columns are for high-resolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL.
• Characteristic: Prepacked format – for convenience and reproducibility; can be used with standalone pump or chromatography system.
  High resolution – for high purity.
  Scalable – separations can be scaled up using Superdex 75 prep grade resin.
  ÄKTA system compatible – Fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems.
• Maximum operating pressure: 0.3 MPa (3 bar, 42 psi)
• Matrix: Cross-linked agarose, spherical
• Average particle size: ~ 34 μm
• Recommended flow rate: 10 cm/h - 50 cm/h
• Recommended column height: 600 mm
• pH working range: 3 to 12
• CIP stability: 1 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Wash the column with one-half to one CV 0.5 M NaOH at a flow rate of 0.8 mL/min for 16/600 or 2.2 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin. After cleaning, immediately equilibrate the column with at least two CV buffer. Further equilibration is necessary if the buffer contains detergents. Wait until the UV baseline stabilizes before starting a new purification.
• Dimensions: 16 × 600 mm
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 73 psi)