HiPrep Butyl FF
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HiPrep Butyl FF

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Hi-131P HiPrep Butyl FF Inquiry
Product Information
Cat#No# Hi-131P
Product Overview HiPrep Butyl FF (High Sub) 16/10, are prepacked Butyl Sepharose 4 Fast Flow columns ready to use for hydrophobic interaction chromatography (HIC). The columns are designed for initial and intermediate purification, removing bulk impurities.
Characteristic 20 mL HIC columns provide fast, preparative separation of proteins and other biomolecules.
Provide high capacity, excellent flow properties, and reliable, reproducible separations.
Butyl Sepharose 4 Fast Flow is a well-established, standard aliphatic HIC resin for capture and intermediate purification.
Compatible with simple pump-based configurations or chromatography system.
Maximum operating pressure 5 bar [0.5 MPa] (72 psi)
Matrix Cross-linked agarose, 6%, spherical
Average particle size ~ 90 µm
Ligand Phenyl
Ligand density ~ 45 µmol/mL resin (high sub)
Recommended flow rate < 300 cm/h
Recommended column height 100 mm
Chemical stability Stable to commonly used aqueous buffers, 1.0 M NaOH58 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
pH working range 3 to 13
CIP stability 2 to 14
Storage 4°C to 30°C, 20% Ethanol
Cleaning-in-place 80 mL of a 1.0 M NaOH (0.01 M NaOH for Phenyl Sepharose HP) solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water.
80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water.
Pack size 20 mL
Maximum flow velocity 10 mL/min (300 cm/h)
Dimensions 16 × 100 mm
Column i.d. 16 mm
Column hardware pressure limit 0.5 MPa (5 bar, 72.5 psi)
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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