HiPrep CM FF

• Cat#No#: Hi-204P
• Product Overview: HiPrep CM FF 16/10 is a weak cation exchanger prepacked with CM Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.
• Characteristic: 20 mL HiPrep columns provide fast, reliable, and reproducible preparative ion exchange separations.
  Good flow rates and high capacity.
  The industry standard for ion exchange chromatography.
  Compatible with single-pump configurations and chromatography systems.
  Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.09 to 0.13 mmol H + /mL resin
• Matrix: Cross-linked 6% agarose, spherical
• Ionic Exchanger Type: Weak cation
• Average particle size: ~ 90 µm
• Dynamic binding capacity: ~ 50 mg Ribonuclease A/mL resin
• Recommended flow rate: < 300 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1 M NaOH7 , 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 80 mL of a 2 M NaCl solution (removes ionically bound proteins from the column) followed by 50 mL distilled water.
  80 mL of a 1.0 M NaOH solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water.
  80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water.
• Pack size: 20 mL
• Maximum flow velocity: 300 cm/h (10 mL/min)
• Dimensions: 16 × 100 mm
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72.5 psi)
• Functional group: -O-CH2COO-