HiPrep Heparin FF columns

• Cat#No#: Hi-062P
• Product Overview: HiPrep Heparin FF columns are well suited for capture or intermediate purification of proteins with affinity for heparin.
• Description: HiPrep Heparin FF 16/10 is a prepacked ready to use column. The column provides fast, preparative separations of proteins and other biomolecules based on their affinity for heparin. Heparin is a naturally occurring glycosaminoglycan which is an effective affinity binding and ion exchange ligand for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin is coupled to Sepharose 6 Fast Flow with a chemically optimized linkage.
• Characteristic: Suitable for plasma protein purification of various proteins, including antithrombin III antibodies and coagulation factors.
  Prepacked with Heparin Sepharose Fast Flow resin.
  Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment .
  HiPrep column format: optimized for convenient purification scale-up.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Sample preparation: Dissolve the sample in binding buffer, filter through 0.45 µm filter or centrifuge at 10 000 × g for 10 minutes.
• Ligand Coupling Method: Reductive amination
• Matrix: cross-linked agarose, 6%, spherical
• Average particle size: ~ 90 µm
• Ligand: Heparin (procine origin)
• Ligand density: ~ 2 mg heparin/mL resin
• Recommended flow rate: 2–10 mL/min (60-300 cm/h)
• Recommended column height: 100 mm
• pH working range: 4 to 12
• CIP stability: 4 to 13
• Storage: 4 to 30°C, 0.05 M Sodium Acetate containing 20% Ethanol
• Cleaning-in-place: Wash the column with 40 mL of 2 M NaCl at a flow rate of 5 mL/min at room temperature to remove ionically bound proteins.
• Pack size: 20 mL
• Maximum flow velocity: 10 mL/min (300 cm/h)
• Maximum operating backpressure: 0.15 MPa (1.5 bar, 22 psi)
• Dimensions: 16 × 100 mm
• Column volume: 20 ml
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 73 psi)