Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Hi-132P | HiPrep Octyl FF | Inquiry |
Product Information | |
Cat#No# | Hi-132P |
Product Overview | HiPrep Octyl FF (High Sub) 16/10, are prepacked Octyl Sepharose 4 Fast Flow columns ready to use for hydrophobic interaction chromatography (HIC). The columns are designed for initial and intermediate purification, removing bulk impurities. |
Characteristic | 20 mL HIC columns provide fast, preparative separation of proteins and other biomolecules. Provide high capacity, excellent flow properties, and reliable, reproducible separations. Octyl Sepharose 4 Fast Flow is a well-established, standard aliphatic HIC resin for capture and intermediate purification. Compatible with simple pump-based configurations or chromatography system. The octyl derivative adds a different and complementary selectivity to the Sepharose Fast Flow HIC product family. |
Maximum operating pressure | 5 bar [0.5 MPa] (72 psi) |
Matrix | Cross-linked agarose, 4%, spherical |
Average particle size | ~ 90 µm |
Ligand | Octyl |
Ligand density | ~ 5 µmol/mL resin |
Recommended flow rate | < 300 cm/h |
Recommended column height | 100 mm |
Chemical stability | Stable to commonly used aqueous buffers, 1.0 M NaOH5 , 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol. |
pH working range | 3 to 13 |
CIP stability | 2 to 14 |
Storage | 4°C to 30°C, 20% Ethanol |
Cleaning-in-place | 80 mL of a 1.0 M NaOH (0.01 M NaOH for Phenyl Sepharose HP) solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water. 80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water. |
Pack size | 20 mL |
Maximum flow velocity | 10 mL/min (300 cm/h) |
Dimensions | 16 × 100 mm |
Column i.d. | 16 mm |
Column hardware pressure limit | 0.5 MPa (5 bar, 72.5 psi) |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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