HiPrep Phenyl FF (High Sub)

• Cat#No#: Hi-133P
• Product Overview: HiPrep Phenyl FF (High Sub) 16/10, are prepacked Phenyl Sepharose 6 Fast Flow columns ready to use for hydrophobic interaction chromatography (HIC). The columns are designed for initial and intermediate purification steps, removing bulk impurities.
• Characteristic: 20 mL HIC columns provide fast, preparative separation of proteins and other biomolecules.
  Provide high capacity, excellent flow properties, and reliable, reproducible separations.
  Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC resin for capture and intermediate purification requiring low to medium hydrophobicity.
  Two levels of substitution help find optimal selectivity and binding capacity for a given application.
  Compatible with simple pump-based configurations or chromatography system.
• Maximum operating pressure: 5 bar [0.5 MPa] (72 psi)
• Matrix: Cross-linked agarose, 6%, spherical
• Average particle size: ~ 90 µm
• Ligand: Phenyl
• Ligand density: ~ 45 µmol/mL resin (high sub)
• Recommended flow rate: 5 mL/min (150 cm/h)
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH58 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 2 to 14
• Storage: 4°C to 30°C, 20% Ethanol
• Cleaning-in-place: 80 mL of a 1.0 M NaOH (0.01 M NaOH for Phenyl Sepharose HP) solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water.
  80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water.
• Pack size: 20 mL
• Maximum flow velocity: 10 mL/min (300 cm/h)
• Dimensions: 16 × 100 mm
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72.5 psi)