HiPrep Phenyl HP

• Cat#No#: Hi-134P
• Product Overview: HiPrep 16/10 Phenyl HP is a prepacked column designed for preparative hydrophobic interaction chromatography (HIC) with good resolution.
• Description: Phenyl Sepharose HP chromatography resin is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic resin with no ionic properties.
• Characteristic: Aromatic HIC resin for intermediate purification and polishing.
  Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
  Provides fast, easy high-capacity separations in a convenient format.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Matrix: Cross-linked agarose, spherical
• Average particle size: ~ 34 µm
• Ligand: Phenyl
• Ligand density: ~ 25 µmol/mL resin
• Recommended flow rate: < 150 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 0.01 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 12
• CIP stability: 3 to 12
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
• Cleaning-in-place: 80 mL of a 1.0 M NaOH (0.01 M NaOH for Phenyl Sepharose HP) solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water.
  80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water.
• Pack size: 20 mL
• Maximum flow velocity: 5 mL/min (150 cm/h)
• Dimensions: 16 × 100 mm
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72.5 psi)