HiPrep Sephacryl S-200 HR

• Cat#No#: Hi-313P
• Product Overview: HiPrep Sephacryl S-200 HR (high-resolution) size exclusion chromatography columns are designed for routine protein purification at laboratory scale.
• Characteristic: Broad fractionation range: Mr ~ 5 000 to ~ 250 000 (globular proteins), Mr ~ 1 000 to ~ 80 000 (dextrans) suitable for varied applications, including purification of recombinant tagged proteins.
  High capacity columns: Preparative separation of protein in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively.
  Convenient: Pre-packed Sephacryl columns are easily connected to ÄKTA systems and other chromatography systems for automated chromatography at low to medium pressure.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Matrix: Cross-linked copolymer of allyl dextran and N,N-methylenebisacrylamide.
• Particle Size: 25–75 μm
• Average particle size: 47 μm
• Recommended flow rate: 15 cm/h
• Recommended column height: 600 mm
• pH working range: 3 - 11
• CIP stability: 2 - 13
• Storage: 4°C to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Wash the column with one-half to one CV 0.5 M NaOH at a flow rate of 0.8 mL/min for 16/600 or 2.2 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin. After cleaning, immediately equilibrate the column with at least two CV buffer. Further equilibration is necessary if the buffer contains detergents. Wait until the UV baseline stabilizes before starting a new purification.
• Maximum flow velocity: 1.0 mL/min
• Dimensions: 16 × 600 mm
• Column volume: 120 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa, 5 bar, 73 psi