HiPrep Sephacryl S-500 HR

• Cat#No#: Hi-315P
• Product Overview: HiPrep Sephacryl S-500 HR columns are designed for preparative size exclusion chromatography of large macromolecules e.g. viruses or group separation of DNA restriction fragments.
• Characteristic: Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  Gel filtration columns for low to medium pressure ÄKTA systems.
  Well-established in industrial production.
  The HiPrep Sephacryl series offer reproducible separations over a wide molecular weight range.
  Convenient, prepacked HiPrep Sephacryl columns speed up protein purification.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Matrix: Cross-linked copolymer of allyl dextran and N,N-methylenebisacrylamide.
• Particle Size: 25–75 μm
• Average particle size: 47 μm
• Recommended flow rate: 15 cm/h
• Recommended column height: 600 mm
• pH working range: 3 - 11
• CIP stability: 2 - 13
• Storage: 4°C to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Wash the column with one-half to one CV 0.5 M NaOH at a flow rate of 0.8 mL/min for 16/600 or 2.2 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin. After cleaning, immediately equilibrate the column with at least two CV buffer. Further equilibration is necessary if the buffer contains detergents. Wait until the UV baseline stabilizes before starting a new purification.
• Maximum flow velocity: 1.0 mL/min
• Dimensions: 16 × 600 mm
• Column volume: 120 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa, 5 bar, 73 psi