HiPrep SP FF Cation Exchange Column

• Cat#No#: Hi-209P
• Product Overview: HiPrep SP FF is a 20 mL strong cation exchange chromatography column for small scale protein purification.
• Description: HiPrep 16/10 columns for ion exchange (IEX) chromatography are prepacked 20 mL cation and anion exchange columns for preparative separations of proteins and other biomolecules based on charge.
• Characteristic: Packed with SP Sepharose Fast Flow strong cation exchange resin.
  Convenient prepacked ion exchange chromatography column.
  Fast, reliable, and reproducible preparative ion exchange separation.
  Feature good flow rates and high capacity.
  20 mL HiPrep column format compatible with single-pump configurations and chromatography systems.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Metal ion capacity: 0.18 to 0.25 mmol H+ /mL resin
• Matrix: Cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Strong cation
• Average particle size: ~ 90 µm
• Dynamic binding capacity: ~ 70 mg ribonuclease A/mL resin
• Recommended flow rate: < 300 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH , 8 M urea, 6 M guanidine hydrochloride, and 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
• Pack size: 20 mL
• Dimensions: 16 × 100 mm
• Column volume: 20 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 73 psi)
• Functional group: -CH2- CH2-CH2SO3 -