HiPrep SP HP

• Cat#No#: Hi-210P
• Product Overview: HiPrep SP HP 16/10 is a strong cation exchanger designed for preparative ion exchange chromatography separations. This 20 mL column is prepacked with SP Sepharose High Performance chromatography resin. The resin is based on rigid, highly cross-linked beaded agarose with a mean bead diameter of 34 µm for high resolution separations.
• Description: High mechanical strength of the matrix allows for high flow rates, together with outstanding resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.
• Characteristic: For high-performance preparative scale separations where high efficiency and high resolution are desired.
  Highly cross-linked agarose provides excellent chemical and physical stability.
  Slim, modern design allows for easy handling and connection.
• Maximum operating pressure: 0.3 MPa (44 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.15 to 0.20 mmol H + /mL resin
• Matrix: Cross-linked agarose, spherical
• Ionic Exchanger Type: Strong cation
• Average particle size: ~ 34 µm
• Dynamic binding capacity: ~ 55 mg Ribonuclease A/mL resin
• Recommended flow rate: < 150 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1 M NaOH6 , 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
• Cleaning-in-place: 80 mL of a 2 M NaCl solution (removes ionically bound proteins from the column) followed by 50 mL distilled water.
  80 mL of a 1.0 M NaOH solution (removes precipitated proteins, hydrophobically bound proteins, and lipoproteins from the column) followed by 80 mL distilled water.
  80 mL of 70% ethanol or 30% isopropanol (removes proteins, lipoproteins, and lipids that are strongly hydrophobically bound to the column) followed by 60 mL distilled water.
• Pack size: 20 mL
• Maximum flow velocity: 150 cm/h (5 mL/min)
• Dimensions: 16 × 100 mm
• Column volume: 20 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72.5 psi)
• Functional group: -CH2CH2CH2SO3 -