His GraviTrap

• Cat#No#: His-102P
• Product Overview: His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
• Description: His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). The column gives fast and simple gravity-flow purifications without any need for a purification system. Large sample volumes can be applied in one go, and the histidine-tagged protein is effectively eluted in a small volume.
  His GraviTrap contains Ni Sepharose 6 Fast Flow medium, which has negligible nickel leakage and is compatible with denaturing and reducing agents as well as a wide range of additives. The columns are made of biocompatible polypropylene. Special frits protect the medium from running dry during purification.
• Characteristic: For fast and simple purification of histidine-tagged* proteins.
  Convenient, prepacked gravity-flow column.
  Allows direct purification of both clarified and unclarified cell lysates.
  High binding capacity; approximately 40 mg of pure protein per column.
  Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
• Average particle size: 90 µm
• Dynamic binding capacity: Approx. 40 mg histidine-tagged protein/column.
• Chemical compatibility: Stable in all commonly used buffers, reducing agents, denaturants, and detergents.
• pH working range: 2 to 14
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 10 × 1 mL
• Material: Polypropylene barrel, polyethylene frits