His GraviTrap kit

• Cat#No#: His-104P
• Product Overview: The benefits of His GraviTrap and His Buffer Kit are combined in His GraviTrap Kit, which contains two packs of His GraviTrap and one pack of His Buffer Kit. His GraviTrap Kit contains columns and buffers for 20 purifications.
• Description: His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). The column gives fast and simple gravity-flow purifications without any need for a purification system. Large sample volumes can be applied in one go, and the histidine-tagged protein is effectively eluted in a small volume.
  His GraviTrap contains Ni Sepharose 6 Fast Flow medium, which has negligible nickel leakage and is compatible with denaturing and reducing agents as well as a wide range of additives. The columns are made of biocompatible polypropylene. Special frits protect the medium from running dry during purification.
• Characteristic: Short purification times, approx. 30 min.
  High protein binding capacity, ≈ 40 mg/column.
  Purifies unclarified samples.
• Applications: For fast and simple purification of histidine-tagged* proteins.
• Average particle size: 90 µm
• Dynamic binding capacity: Approx. 40 mg histidine-tagged protein/column.
• Chemical stability: Stable in all commonly used buffers, reducing agents, denaturing agents and detergents.
• CIP stability: 2 to 14
• Storage: 4 - 30°C, 20% Ethanol
• Pack size: 10 columns
• Kit contents: 2 packs of His GraviTrap and 1 pack of His Buffer Kit.
• Material (column hardware): Polypropylene (PP) barrel, Polyethylene (PE) frits