His GraviTrap TALON

• Cat#No#: His-103P
• Product Overview: His GraviTrap TALON is a prepacked gravity flow column for convenient manual purification of histidine-tagged proteins.
• Description: His GraviTrap TALON gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged proteins directly from clarified or unclarified cell lysates. His GraviTrap TALON columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography resin from running dry during purification.
• Characteristic: Convenient histidine-tagged purification with high purity (> 90%) and binding capacity of up to 15 mg/column.
  Ready-to-use gravity flow column prepacked with 1 mL TALON Superflow cobalt-IMAC (immobilized metal affinity chromatography) resin offering a different selectivity compared to nickel-charged resins.
  One run takes about 30 minutes, and large sample volumes can be applied all at once.
• Particle Size: 60 to160 μm
• Dynamic binding capacity: up to 15mg histidine-tagged protein
• pH working range: 2 to 14
• CIP stability: 3 to 12
• Pack size: 10
• Material: Polypropylene barrel, polyethylene frits