His MultiTrap HP

• Cat#No#: Hi-399C
• Product Overview: His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
• Description: His MultiTrap HP are prepacked, 96-well filter plates for screening and small-scale, high-throughput parallel purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Consistent well-to-well and plate-to-plate performance ensures high reproducibility. The ability to load unclarified lysates on His MultiTrap plates simplifies the workflow and saves time. The standardized 96-well plate format gives great flexibility, both when working with automated robotic systems and when manually using centrifugation or vacuum. Using the filter plates, 96 samples with up to 1 mg of histidine-tagged protein/well can be purified in 60 min.
• Characteristic: Highly reproducible well-to-well and plate-to-plate results.
  Less sample pretreatment needed — load unclarified sample directly, increase reproducibility in results, and save time.
  Easy and predictable scale-up to HisTrap FF, HisPrep FF 16/10, or HisTrap HP prepacked columns.
  High chemical stability and high binding capacity — purifies up to 1 mg of histidine-tagged protein per well.
  Prepacked with Ni Sepharose media, which have low nickel leakage and are compatible with a wide range of additives used in protein purification.
• Applications: High reproducibility of histidine-tagged protein purification from unclarified and clarified lysates using His MultiTrap.
  Solubility effects of detergents in buffers during purification of membrane proteins.
• Sample preparation: His MultiTrap HP purify histidine-tagged proteins directly from unclarified cell lysates. No centrifugation or filtration is needed prior to loading the wells. Samples are prepared by straightforward chemical and/or mechanical lysis. If the sample is viscous, simply extend the mechanical treatment. A general sample preparation protocol involves: (1) suspending the cells/cell paste, (2) enzymatic lysis using lysozyme, DNAse I, and adding MgCl2 etc., (3) mechanical lysis by sonication, homogenization, or freeze/thaw, (4) adjusting pH, and (5) applying unclarified lysate directly to the wells.
• Metal ion capacity: ~15 μmol Ni²+ /mL medium
• Average particle size: 34 μm
• Dynamic binding capacity: Up to 1 mg histidine-tagged protein/well
• Chemical stability: 0.01 M HCl, 0.1 M NaOH (tested for one week at 40°C); 1 M NaOH or 70% acetic acid (tested for 12 h) 2% SDS (tested for 1 h); 30% 2-propanol (tested for 30 min).
• Chemical compatibility: Stable in all commonly used buffers, reducing agents, denaturants, and detergents.
• pH working range: 2–14
• CIP stability: 3–12
• Storage: 4 - 30°C, 20% Ethanol
• Pack size: 4 × 96-well filter plates
• Column volume: 800 μL