Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Hi-400C | His MultiTrap TALON | Inquiry |
Product Information | |
Cat#No# | Hi-400C |
Product Overview | His MultiTrap TALON is a ready-to-use 96-well filter plate for high reproducibility in expression screening and small-scale parallel purification of histidine-tagged proteins. |
Description | His MultiTrap TALON multiwell plates are designed for high-throughput screening of histidine-tagged proteins from clarified cell lysates. His MultiTrap TALON prepacked plates are manufactured with high reproducibility with regards to the well and resins volumes in order to enable consistent results. The average purity of His MultiTrap TALON is > 85% with yields up to 1 mg/well. Leakage of cobalt ions from TALON Superflow resin is negligible. His MultiTrap TALON is used manually with vacuum or centrifugation, or in a liquid handling station. |
Characteristic | Up to 1 mg of pure histidine-tagged protein per well. Prefilled with 50 µL TALON Superflow cobalt-IMAC (immobilized metal affinity chromatography) resin for reproducible and high throughput parallel screening, offering a different selectivity compared to nickel-charged resins. Large sample volumes can be applied all at once. |
Particle Size | 60 to160 μm |
Dynamic binding capacity | Up to 1 mg histidine-tagged protein/ well |
Chemical compatibility | Stable in all commonly used buffers, reducing agents, denaturants, and detergents. |
pH working range | 2–14 |
CIP stability | 3–12 |
Storage | 4 to 8°C, 20% Ethanol |
Pack size | 4 × 96-well filter plates |
Column volume | 800 μl |
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