His SpinTrap TALON

• Cat#No#: His-107P
• Product Overview: His SpinTrap TALON is a single-use column for rapid screening with small samples of histidine-tagged proteins.
• Description: His SpinTrap TALON is designed for high purity, small-scale purification and screening of histidine-tagged proteins from clarified cell lysates. His SpinTrap TALON columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from TALON Superflow resin is negligible.
• Characteristic: Suitable for purification of multiple samples in parallel, such as screening.
  Convenient, ready-to-use single-use spin column, prepacked with 100 μL TALON Superflow cobalt-IMAC (immobilized metal affinity chromatography) resin offering a different selectivity compared to nickel-charged resins.
  With a microcentrifuge, one run takes about 10 minutes.
• Particle Size: 60 to160 μm
• Dynamic binding capacity: Approximately 1 mg histidine-tagged protein/column.
• Chemical stability: Stable in all commonly used buffers, denaturants and detergents.
• pH working range: 2 to 14
• CIP stability: 3 to 12
• Storage: 4°C to 8°C
• Pack size: 50 × 100 µL
• Dimensions: 127.8 × 85.5 × 30.6 mm
• Number of wells: 96
• Material: Polypropylene barrel, polyethylene frits
• Centrifugation force recommended: 100 to 500 × g
• Centrifugation force max.: 700 × g