His-tagged column package, 2-step protocol

• Cat#No#: His-108P
• Product Overview: The column package allows you to combine affinity with size exclusion chromatography to obtain highly pure his-tagged proteins.
• Description: His-tags are widely used because they are small and rarely interfere with the function, activity, or structure of target proteins. All his-tagged protein purification protocols typically start with an affinity chromatography (IMAC = immobilized metal ion affinity chromatography) step. It enables the isolation of the target his-tagged protein from initial sample (e.g., lysate, cell culture). After the first affinity chromatography step is run on an IMAC column, the purity level is usually moderate (> 80%).
• Characteristic: Contains a HisTrap HP column 1 mL prepacked with Ni Sepharose High Performance (HP) affinity resin for preparative purification of his-tagged proteins.
  You achieve final purity with HiPrep 16/60 Sephacryl S-200 HR size exclusion chromatography column, which removes any remaining trace of impurities or closely related substances and imidazole.
  Includes instructions with guidance and tips showing you how to get started.
• Applications: Purification of his-tagged proteins
• Storage: 4°C to 30°C
• Pack size: 1 kit