HiScreen Blue Sepharose FF

• Cat#No#: Hi-064P
• Product Overview: HiScreen columns prepacked with Blue Sepharose Fast Flow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
• Description: HiScreen columns are prepacked with a range of BioProcess chromatography resins (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption. The resins used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
• Characteristic: Can be used for depletion of albumin.
  Prepacked, ready-to-use columns for convenient process development.
  Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  Easy connection in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Matrix: Cross-linked agarose, 6%, spherical
• Average particle size: ~ 90 µm
• Ligand density: 6.7 to 7.9 μmol Cibacron Blue/mL resin
• Dynamic binding capacity: ~ 24 mg HSA/mL resin
• Recommended flow rate: < 600 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH7 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 4 to 12
• CIP stability: 3 to 13
• Storage: 4 to 8°C, 20% Ethanol, 0.1 M potassium phosphate
• Cleaning-in-place: Precipitated proteins: 1. Wash the column with 4 column volumes (CV) of either 0.5 M (HiScreen Capto Blue) or 0.1 M NaOH (HiScreen Blue FF) at 40 cm/h. 2. Wash with 3 to 4 CV of 70% ethanol or 2 M potassium thiocyanate. 3. Wash immediately with at least 5 CV filtered start buffer, pH 8.0. or 1. Wash the column with 2 CV of 6 M guanidine hydrochloride. 2. Wash immediately with at least 5 CV filtered start buffer, pH 8.0.
  Strongly bound hydrophobic proteins, lipoproteins, and lipids: 1. Wash the column with 3 to 4 CV of up to 70% ethanol or 30% isopropanol.. 2. Wash immediately with at least 5 CV filtered start buffer, pH 8.0. or 1. Wash with 2 CV detergent in a basic or acidic solution, e.g., 0.1% non-ionic detergent in 1 M acetic acid. Wash at a flow rate of 40 cm/h. 2. Remove residual detergent by washing with 5 CV of 70% ethanol. 3. Wash immediately with at least 5 CV filtered start buffer, pH 8.0.
• Pack size: 4.7 mL
• Maximum flow velocity: 3.2 mL/min
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)